Journal: Veterinary Sciences
Article Title: Intra- and Intertumoral Microglia/Macrophage Infiltration and Their Associated Molecular Signature Is Highly Variable in Canine Oligodendroglioma: A Preliminary Evaluation
doi: 10.3390/vetsci10060403
Figure Lengend Snippet: TGFβ and galectin-3 signaling may be relevant in canine oligodendroglioma. ( A ) Representative immunoblots for proteins within the canonical transforming growth factor β (TGFβ) pathway and galectin-3 (GAL-3) in normal canine brain (n = 3) and HGG homogenates (n = 4). Relative protein expression of ( B ) TGFβ targets or ( C ) GAL-3 ( p = 0.0608) was not different between normal brain (n = 3) and HGG homogenate (n = 4). Comparisons based on unpaired t -test; bars represent group mean with standard error of the mean. ( D ) Representative immunofluorescence staining for GAL-3 in HGG. Multifocal areas of robust cytoplasmic GAL-3 IR were observed to co-localize with OLIG2 IR (top panel). Serial sections of tumor areas rich in IBA-1 IR had weak GAL-3 IR without co-localization with IBA-1-positive cells (bottom panel). Scale bars, 5 μm.
Article Snippet: Primary antibodies utilized included: microglia/macrophages: rabbit polyclonal anti-ionized calcium-binding adaptor molecule (IBA-1) (Wako Pure Chemical Industries, Ltd., Chuo-Ku, Osaka, Japan; 019-19741, 1:1000); oligodendroglioma cells: rabbit polyclonal anti-oligodendrocyte transcription factor 2 (OLIG2) (Millipore Sigma, Burlington, MA, USA; AB9610, 1:200); galectin-3 (GAL-3): mouse monoclonal anti-GAL-3 (Abcam, Boston, MA, USA; ab31707, 1:1000).
Techniques: Western Blot, Expressing, Immunofluorescence, Staining
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